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KMID : 0616120230540040191
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Korean Journal of Pharmacognosy
2023 Volume.54 No. 4 p.191 ~ p.196
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Simultaneous Quantitative Analysis of Echinacoside, Poliumoside, Isoacteoside and Acacetin-diglucuronide from the Leaf of Callicarpa dichotoma (Lour.) K. Koch
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Kim Eun-Nam
Lee Da-Eun
Lee Won-Jin
Kim Tae-Yeon
Roh Yooon-Seok
Jeong Gil-Saeng
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Abstract
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Callicarpa dichotoma (Lour.) K. Koch has a number of ingredients that are recognized to have physiological activity in hematuria, rheumatism, inflammation, and other conditions. Although study on the contents of C. dichotoma has not yet been done, it is known that the principal constituents are chemicals from the flavonoid and phenylethanoid glycoside class. Consequently, in this work, the amounts of echinacoside, poliumoside, isoacteoside and acacetin-diglucuronide found in the C. dichotoma leaf extract were measured utilizing a diode array detector in high-performance liquid chromatography (HPLC). HPLC was used to carry out the content analysis of each component, and tests for accuracy, specificity, and precision were used to validate the analysis technique. The result showed that the calibration curves of the four compounds, echinacoside, poliumoside, isoacteoside and acacetin-diglucuronide, a large linearity with a correlation coefficient (R²) of 0.9935, 0.9909, 0.9906 and 0.9934. Intra and inter day measurement accuracy of the four compounds was 94.56 to 117.77% and showed precision was less than 3%. Therefore, content analysis showed that echinacoside (1.75±0.28%), poliumoside (4.82±0.66%), isoacteoside (1.40±0.18%) and acacetin-diglucuronide (6.38±0.11%).
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KEYWORD
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Callicarpa dichotoma, Echinacoside, Poliumoside, Isoacteoside, Acacetin-diglucuronide
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